Search results for "Amaryllidaceae Alkaloids"

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Interactions between the yeast mitochondrial and nuclear genomes: isogenic suppressive and hypersuppressive petites differ in their resistance to the…

1990

In a previous paper we have shown that the alkaloid lycorine inhibits growth of rho+, mit- and rho-, strains of Saccharomyces cerevisiae, whereas strains devoid of mitochondrial DNA (rho degrees) are resistant to more than 200 micrograms/ml of the alkaloid. In this report we show that hypersuppressive petites are almost as resistant as rho degrees mutants, whereas isogenic rho- petites, which have retained longer segments of the genome, are sensitive to the drug.

GeneticsMitochondrial DNAMutationbiologyAlkaloidSaccharomyces cerevisiaeMutantDrug Resistance MicrobialSaccharomyces cerevisiaeGeneral Medicinebiology.organism_classificationLycorinemedicine.disease_causeDNA MitochondrialMolecular biologyGenomeYeastPhenanthridineschemistry.chemical_compoundchemistryAmaryllidaceae AlkaloidsGeneticsmedicineDNA FungalCurrent Genetics
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Cytotoxicity of ungeremine towards multi-factorial drug resistant cancer cells and induction of apoptosis, ferroptosis, necroptosis and autophagy

2018

Abstract Background Successful cancer chemotherapy is hampered by resistance of cancer cells to established anticancer drugs. Numerous natural products reveal cytotoxicity towards tumor cells. Purpose The present study was aimed to determine the cytotoxicity of a betaine-type alkaloid, ungeremine, towards 9 cancer cell lines including various sensitive and drug-resistant phenotypes. The mode of action of this compound was further investigated. Methods The cytotoxicity, ferroptotic and necroptotic cell death were determined by the resazurin reduction assay. Caspase activation was evaluated using the caspase-Glo assay. Flow cytometry was applied for the analysis of cell cycle analysis (PI sta…

Programmed cell deathNecroptosisPharmaceutical ScienceApoptosis03 medical and health sciencesAlkaloids0302 clinical medicineAnnexinCell Line TumorDrug DiscoveryAutophagyHumansCytotoxic T cellCytotoxicity030304 developmental biologyPharmacology0303 health sciencesCell DeathPlant ExtractsChemistryUngeremineIndolizinesAntineoplastic Agents PhytogenicDrug Resistance MultipleComplementary and alternative medicineDrug Resistance NeoplasmApoptosis030220 oncology & carcinogenesisCancer cellAmaryllidaceae AlkaloidsCancer researchMolecular MedicineReactive Oxygen SpeciesPhytomedicine
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